کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10159405 | 48 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Young developmental age cardiac extracellular matrix promotes the expansion of neonatal cardiomyocytes in vitro
ترجمه فارسی عنوان
ماتریکس خارج سلولی قلبی جوان در دوران رشد، گسترش سلول های قلبی نوزاد را در شرایط آزمایشگاهی گسترش می دهد
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کلمات کلیدی
مهندسی بافت قلب، قلب و عروق، ترویج ماتریکس غیر سلولی، تصویربرداری نسل دوم هارمونی،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
A major limitation to cardiac tissue engineering and regenerative medicine strategies is the lack of proliferation of postnatal cardiomyocytes. The extracellular matrix (ECM) is altered during heart development, and studies suggest that it plays an important role in regulating myocyte proliferation. Here, the effects of fetal, neonatal and adult cardiac ECM on the expansion of neonatal rat ventricular cells in vitro are studied. At 24Â h, overall cell attachment was lowest on fetal ECM; however, â¼80% of the cells were cardiomyocytes, while many non-myocytes attached to older ECM and poly-l-lysine controls. After 5Â days, the cardiomyocyte population remained highest on fetal ECM, with a 4-fold increase in number. Significantly more cardiomyocytes stained positively for the mitotic marker phospho-histone H3 on fetal ECM compared with other substrates at 5Â days, suggesting that proliferation may be a major mechanism of cardiomyocyte expansion on young ECM. Further study of the beneficial properties of early developmental aged cardiac ECM could advance the design of novel biomaterials aimed at promoting cardiac regeneration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Biomaterialia - Volume 10, Issue 1, January 2014, Pages 194-204
Journal: Acta Biomaterialia - Volume 10, Issue 1, January 2014, Pages 194-204
نویسندگان
C. Williams, K.P. Quinn, I. Georgakoudi, L.D. III,