کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10160505 | 124 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heparin functionalized PEG gels that modulate protein adsorption for hMSC adhesion and differentiation
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Heparin was modified with methacrylate groups, copolymerized with dimethacrylated poly(ethylene glycol), and analyzed as a localized delivery vehicle for bFGF and synthetic extracellular matrix for the differentiation of hMSCs. By deriving cues from molecules normally present in the extracellular matrix (ECM), a complex network of collagens, laminin, fibronectin, glycosaminoglycans, and growth factors, synthetic cell scaffolds can be designed that actively sequester important bioactive signals. Among the glycosaminoglycans, heparin binds reversibly with many proteins, therefore, poly(ethylene glycol) based biomaterials, normally resistant to cell adhesion, functionalized with heparin in order to sequester important proteins, can actively and selectively stimulate desired cell functions. Results demonstrate that methacrylate-modified heparin retained its ability to bind heparin-binding proteins both in solution and when copolymerized with dimethacrylated PEG in a hydrogel. In addition, the heparin functionalized gels can deliver biologically active bFGF for up to 5 weeks. Finally, the gels were examined as a potential scaffold for hMSC culture and were found to promote adhesion, proliferation, and osteogenic differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Biomaterialia - Volume 1, Issue 4, July 2005, Pages 461-470
Journal: Acta Biomaterialia - Volume 1, Issue 4, July 2005, Pages 461-470
نویسندگان
Danielle S.W. Benoit, Kristi S. Anseth,