کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10160599 195 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetic study of 7-O-ethyl ganoderic acid O stability and its importance in the preparative isolation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Kinetic study of 7-O-ethyl ganoderic acid O stability and its importance in the preparative isolation
چکیده انگلیسی
Ganoderic acid (GA) is a kind of important bioactive triterpenoid with anti-cancer effect. 7-O-Ethyl ganoderic acid O (7-O-ethyl GA-O) was recently found to be a promising target for mass production of bioactive individual GA due to its high content. However, its apparent degradation was observed during preparation procedures. In this research, to understand and control its degradation process during the preparative isolation, a systematic kinetic investigation on 7-O-ethyl GA-O stability was done not only under different conditions of pH and temperature, but in different solvents as well. 7-O-Ethyl GA-O was proved to possess an optimal stability in neutral and non-polar environment. Its degradation kinetics and thermodynamics could be effectively described by first-order reaction model and Arrhenius equation. The activation energy was estimated to be 38.2 ± 3.6 kJ/mol in acidic methanol (0.01 mM HCl, pH = 5.1), 103.2 ± 2.4 kJ/mol in aqueous methanol (20% water), and 105.8 ± 3.3 kJ/mol in methanol. Furthermore, the possible mechanism of acid-catalyzed 7-O-ethyl GA-O decomposition in methanol was proposed, consisting of a fast protonation, followed by a committed step of ethanol removal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 53, Issue 2, 15 January 2011, Pages 182-186
نویسندگان
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