Self-interaction chromatography of proteins on a microfluidic monolith

▶ The osmotic second virial coefficient B22 is a measure of protein interactions. ▶ B22 can be measured efficiently by self-interaction chromatography (SIC). ▶ Microfluidic SIC measurements in 100 μm diameter monoliths are demonstrated. ▶ This miniaturized system makes B22 measurement possible with minimal protein consumption.