کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10160673 252 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Operational stability of subtilisin CLECs in organic solvents in repeated batch and in continuous operation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Operational stability of subtilisin CLECs in organic solvents in repeated batch and in continuous operation
چکیده انگلیسی
The operational stability of cross-linked crystals (CLECs) of subtilisin Carlsberg in organic media is compared between repeated batch and continuous flow operation. The study was designed to create similar conditions in the two modes, although these can never be identical in all respects. The biocatalyst inactivates rapidly during the first two batches of 2 h each, losing 50% or more of its activity. The inactivation becomes slower over the following longer batches of 24 h. This pattern of rapid and slower phases of inactivation is similar to that observed in continuous reactors. However, in both acetonitrile and tetrahydrofuran, inactivation is somewhat faster over the first 4 h of continuous operation than in the equivalent batch mode.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 24, Issue 1, 15 May 2005, Pages 11-15
نویسندگان
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