کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10160850 | 268 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
How CTAB assists the refolding of native and recombinant lysozyme
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: How CTAB assists the refolding of native and recombinant lysozyme How CTAB assists the refolding of native and recombinant lysozyme](/preview/png/10160850.png)
چکیده انگلیسی
Refolding of native and recombinant lysozyme was accomplished by dilution with refolding buffer containing low-concentration cetyltrimethylammonium bromide (CTAB). In both cases, the use of β-cyclodextrin (β-CD) was unnecessary. Centered on the interaction between CTAB and the protein being refolded, experimental studies were conducted to investigate the effect of CTAB on the refolding yield, product distribution and the refolding kinetics. A comparative study of the artificial chaperone-assisted refolding of native and recombinant lysozyme was included to generate a more comprehensive understanding of the function of CTAB in assisting protein refolding. It was shown that the formation of CTAB-denatured lysozyme complex occurred at the beginning stage of refolding effectively inhibited the formation of aggregate, leading to an improved refolding. The dissociation of this complex occurred, when lysozyme started to fold into its native conformation catalyzed by GSSG/GSH. The use of β-CD facilitated the dissociation the CTAB-protein complex and thus increased the overall rate of lysozyme refolding. The results described by present study are also helpful for the design of surfactant and the optimization of refolding process for different proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 24, Issue 3, July 2005, Pages 269-277
Journal: Biochemical Engineering Journal - Volume 24, Issue 3, July 2005, Pages 269-277
نویسندگان
Jun Wang, Diannan Lu, Ying Lin, Zheng Liu,