کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10161965 | 1114307 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pulmonary Delivery of Proteins Using Nanocomposite Microcarriers
ترجمه فارسی عنوان
تحویل ریوی پروتئین با استفاده از میکرو حامل نانو کامپوزیت
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کلمات کلیدی
نانوذرات، پلیمرهای زیست تخریب پذیر، سیستم های تحویل دارویی پلیمری، اندازه ذرات، تحویل ریوی، تحویل پروتئین و فرمولاسیون، خشک کردن اسپری،
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
چکیده انگلیسی
In this study, Taguchi design was used to determine optimal parameters for the preparation of bovine serum albumin (BSA)-loaded nanoparticles (NPs) using a biodegradable polymer poly(glycerol adipate-co-Ï-pentadecalactone) (PGA-co-PDL). NPs were prepared, using BSA as a model protein, by the double emulsion evaporation process followed by spray-drying from leucine to form nanocomposite microparticles (NCMPs). The effect of various parameters on NP size and BSA loading were investigated and dendritic cell (DC) uptake and toxicity. NCMPs were examined for their morphology, yield, aerosolisation, in vitro release behaviour and BSA structure. NP size was mainly affected by the polymer mass used and a small particle size 500 nm was achieved. High BSA (43.67 ± 2.3 µg/mg) loading was influenced by BSA concentration. The spray-drying process produced NCMPs (50% yield) with a porous corrugated surface, aerodynamic diameter 1.46 ± 141 µm, fine particle dose 45.0 ± 4.7 µg and fine particle fraction 78.57 ± 0.1%, and a cumulative BSA release of 38.77 ± 3.0% after 48 h. The primary and secondary structures were maintained as shown by sodium dodecyl sulphate poly (acrylamide) gel electrophoresis and circular dichroism. Effective uptake of NPs was seen in DCs with > 85% cell viability at 5 mg/mL concentration after 4 h. These results indicate the optimal process parameters for the preparation of protein-loaded PGA-co-PDL NCMPs suitable for inhalation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 104, Issue 12, December 2015, Pages 4386-4398
Journal: Journal of Pharmaceutical Sciences - Volume 104, Issue 12, December 2015, Pages 4386-4398
نویسندگان
Iman Alfagih, Nitesh Kunda, Fares Alanazi, Sarah R. Dennison, Satyanarayana Somavarapu, Gillian A. Hutcheon, Imran Y. Saleem,