کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10162251 | 1114324 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced Mucosal Immune Responses Against Tetanus Toxoid Using Novel Delivery System Comprised of ChitosanâFunctionalized Gold Nanoparticles and Botanical Adjuvant: Characterization, Immunogenicity, and Stability Assessment
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کلمات کلیدی
Immunology - ایمونولوژیTransmucosal drug delivery - تحویل داروی ترانس مغذیPhysical characterization - خصوصیات فیزیکیVaccine adjuvant - داروی واکسنvaccine delivery systems - سیستم های تحویل واکسنAsparagus racemosus - مارماهی مارماهیGold nanoparticle - نانو ذرات طلاin vitro/in vivo correlations (IVIVC) - همبستگی in vitro / in vivo (IVIVC)Chitosan - کیتوسان
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enhanced Mucosal Immune Responses Against Tetanus Toxoid Using Novel Delivery System Comprised of ChitosanâFunctionalized Gold Nanoparticles and Botanical Adjuvant: Characterization, Immunogenicity, and Stability Assessment Enhanced Mucosal Immune Responses Against Tetanus Toxoid Using Novel Delivery System Comprised of ChitosanâFunctionalized Gold Nanoparticles and Botanical Adjuvant: Characterization, Immunogenicity, and Stability Assessment](/preview/png/10162251.png)
چکیده انگلیسی
Approaches based on combined use of delivery systems and adjuvants are being favored to maximize efficient mucosal delivery of antigens. Here, we describe a novel delivery system comprised of chitosanâfunctionalized gold nanoparticles (CsAuNPs) and saponinâcontaining botanical adjuvant; Asparagus racemosus extract (ARE) for oral delivery of tetanus toxoid (TT). A significant increase in TTâspecific IgG (34.53âfold) and IgA (43.75âfold) was observed when TT-CsAuNPs were formulated with ARE (TT-ARE-CsAuNPs). The local IgA immune responses for TT also showed a significant increase (106.5âfold in intestine washes and 99.74âfold in feces) with AREâbased formulations as compared with plain TT group. No effect of ARE was observed on size, charge, and loading properties of CsAuNPs. Additionally, no effect of ARE and CsAuNPs was observed on antigenicity and secondary structure of TT as determined by fluorescence, circular dichroism, and Fourier transform infrared spectroscopy. The stability studies demonstrated excellent stability profile of formulation at recommended storage conditions. The study establishes the possible role of immunomodulatory adjuvants in particulate delivery systems for mucosal delivery of vaccines. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:3448-3456, 2014
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 11, November 2014, Pages 3448-3456
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 11, November 2014, Pages 3448-3456
نویسندگان
Ganesh Barhate, Manish Gautam, Sunil Gairola, Suresh Jadhav, Varsha Pokharkar,