کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10179902 | 1318693 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteome analysis of alfalfa roots in response to water deficit stress
ترجمه فارسی عنوان
تجزیه پروتئین ریشه یونجه در پاسخ به تنش کمبود آب
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کلمات کلیدی
یونجه پروتئوم ریشه، تنش کمبود آب،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
To evaluate the response of alfalfa to water deficit (WD) stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H2O2 content, lipid peroxidation, DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis (2-DE). A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase (APx2) and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid (ABA) biosynthesis, reactive oxygen species (ROS) regulation, transcription/ translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Integrative Agriculture - Volume 15, Issue 6, June 2016, Pages 1275-1285
Journal: Journal of Integrative Agriculture - Volume 15, Issue 6, June 2016, Pages 1275-1285
نویسندگان
Md Atikur Rahman, Kim Yong-Goo, Alam Iftekhar, Gong-she LIU, Lee Hyoshin, Lee Jeung Joo, Lee Byung-Hyun,