کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10227163 | 429 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An integrated multifunctional platform based on biotin-doped conducting polymer nanowires for cell capture, release, and electrochemical sensing
ترجمه فارسی عنوان
یک پلت فرم چند منظوره یکپارچه مبتنی بر نانوسیمهای پلیمری انجام شده با بیوتین، برای ضبط سلول، انتشار و سنجش الکتروشیمیایی
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کلمات کلیدی
تشخیص سرطان، سلول های تومور گردش خون، انجام پلیمر، تحریک الکتریکی، ایمونوسنسور،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Here, we propose an integrated multifunctional system constructed by conductive disulfide-biotin-doped polypyrrole nanowires (SS-biotin-Ppy NWs) for capture, release, and in situ quantification of circulating tumor cells (CTCs). A well-ordered three-dimensional nanowire structure equipped with a monoclonal antibody offers a significant impact on the cell-capture efficiency, as well as on electrical- or glutathione (GSH)-mediated release of the captured cells. In addition, the electrochemical identification/detection of the captured cancer cells can be directly conducted on the same Ppy NW platform by using horseradish peroxidase (HRP)-labeled and anti-EpCAM-conjugated nanoparticles (HRP/anti-EpCAM Ppy NPs), showing very high sensitivity and specificity. The signal amplification can be clearly attributed to the catalytic response resulting from enzymatic reduction of hydrogen peroxide on Ppy NWs, consequently generating a greatly increased amperometric response with a detection range of 10 to 1Â ÃÂ 104Â cells and a detection limit of as low as 10Â cells. Overall, the proposed Ppy NWs not only present a promising platform for effective cell capture and release but also permit cytosensing capability for on-site analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 36, December 2014, Pages 9573-9580
Journal: Biomaterials - Volume 35, Issue 36, December 2014, Pages 9573-9580
نویسندگان
Woo Young Hong, Seung Hyun Jeon, Eun Sook Lee, Youngnam Cho,