کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10231545 | 1207 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds
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کلمات کلیدی
CPFFNTINPOPAAOmpAMPHEPNGPiEDSSOPDALPPGFPFITCCBDCNTHEPESMWCNT4-CP4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid4-Chlorophenol - 4-کلروفنلINa - INAAChE - آهیEthyl parathion - اتیل پاراتیونAcetylcholinesterase - استیل کولین استرازDiazinon - دیازینونcellulose-binding domain - سلولز اتصال دامنهFenitrothion - فریتروتیونfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتLipoprotein - لیپوپروتئینMethyl parathion hydrolase - متیل پاراتیون هیدرولازCarbon nanotube - نانولوله کربنیMulti-wall carbon nanotube - نانولوله کربنی چند دیوارهmap - نقشهIce nucleation - هسته یخMethyl parathion - پاراتیس متیلOuter membrane protein A - پروتئین غشای خارجی Agreen fluorescent protein - پروتئین فلورسنت سبزice nucleation protein - پروتئین هسته ی یخMussel adhesive protein - پروتئین چسبناکCarbon black - کربن سیاه Mesoporous carbon - کربن مرطوبChlorpyrifos - کلرپیریفوسCoumaphos - کومافوسglycosylphosphatidylinositol - گلیکوزیل فسفاتیدیلینوزیتول
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The development of efficient tools is required for the eco-friendly detoxification and effective detection of neurotoxic organophosphates (OPs). Although enzymes have received significant attention as biocatalysts because of their high specific activity, the uneconomic and labor-intensive processes of enzyme production and purification make their broad use in practical applications difficult. Because whole-cell systems offer several advantages compared with free enzymes, including high stability, a reduced purification requirement, and low preparation cost, they have been suggested as promising biocatalysts for the detoxification and detection of OPs. To develop efficient whole-cell biocatalysts with enhanced activity and a broad spectrum of substrate specificity, several factors have been considered, namely the selected strains, the chosen OP-hydrolyzing enzymes, where enzymes are localized in a cell, and which enhancer will assist the expression, function, and folding of the enzyme. In this article, we review the current investigative progress in the development of engineered whole-cell biocatalysts with excellent OP-hydrolyzing activity, a broad spectrum of substrate specificity, and outstanding stability for the detoxification and detection of OPs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Advances - Volume 32, Issue 3, MayâJune 2014, Pages 652-662
Journal: Biotechnology Advances - Volume 32, Issue 3, MayâJune 2014, Pages 652-662
نویسندگان
Chang Sup Kim, Jeong Hyun Seo, Dong Gyun Kang, Hyung Joon Cha,