کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233170 | 42730 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic synthesis of chitooligosaccharides in organic cosolvents
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Diacetyl-chitobiose (GlcNAc)2 was produced exclusively using a Vibrio furnissii chitinase (chi E, 89Â kDa) hydrolysis of chitin treated with dimethylsulfoxide containing lithium chloride. This method represents a novel way for the preparation of colloidal chitin. Continuous diafiltration was used for the efficient recycling of enzyme and synthesis of (GlcNAc)2 on a preparative scale. Chitooligosaccharides were also synthesized in a transglycosylation reaction employing lysozyme from hen-egg white with diacetyl-chitobiose produced by a Vibrio furnissii chitinase (chi E, 89Â kDa) as a substrate in 50% 2-ethoxy ethyl ether co-solvent system. The co-solvent systems studied were triethyl phosphate, 2-ethoxy ethyl ether and acetone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 37, Issue 6, 1 November 2005, Pages 663-668
Journal: Enzyme and Microbial Technology - Volume 37, Issue 6, 1 November 2005, Pages 663-668
نویسندگان
Jung Hae Yoon,