کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233204 | 42734 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thermally reversible inactivation of Taq polymerase in an organic solvent for application in hot start PCR
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Thermally reversible inactivation of Taq polymerase in an organic solvent for application in hot start PCR Thermally reversible inactivation of Taq polymerase in an organic solvent for application in hot start PCR](/preview/png/10233204.png)
چکیده انگلیسی
In the past, Taq polymerase was reversibly inactivated by modification with a dicarboxylic acid anhydride in aqueous media, to enable 'hot start PCR'. However, there are various constraints in using such a method including temperature and concentration. Here we describe an alternative method whereby Taq polymerase may be reversibly inactivated following incubation with an excess of citraconic anhydride at elevated temperatures, in an anhydrous non-protic organic solvent - tert-butyl methyl ether - by first drying the enzyme with a salt or carbohydrate excipient to form an amorphous powder. Reactivation of the enzyme is due to the instability of the chemical modification at low pH following a short incubation in a suitable buffer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 36, Issue 7, 16 May 2005, Pages 947-952
Journal: Enzyme and Microbial Technology - Volume 36, Issue 7, 16 May 2005, Pages 947-952
نویسندگان
Ariel Louwrier, Anne van der Valk,