کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235495 | 45044 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Continuous cultures for alginate production by Azotobacter vinelandii growing at different oxygen uptake rates
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Continuous cultures for alginate production from Azotobacter vinelandii were conducted at different specific oxygen uptake rates (qo2) under oxygen-limited conditions. As a result of varying the agitation rate, the qo2 increased from 2.2 to 6.0 mmol gâ1 hâ1. An increase in qo2 up to 4.8 mmol gâ1 hâ1 resulted in an increase of alginate concentration, as well as in the specific alginate production rate, which can be attributed to that carbon flux was less diverted towards biomass production. A lower alginate molecular weight (869 kDa as compared to 1350 kDa) was obtained by increasing the qo2 from 2.2 to 4.8 mmol gâ1 hâ1 and a higher gene expression of algL (about 8-fold) was observed, which could explain the decrease in the alginate molecular weight. The results showed for the first time that alginate production and carbon distribution are affected by the qo2. The manipulation of this parameter could be used as a control strategy for alginate production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 46, Issue 9, September 2011, Pages 1879-1883
Journal: Process Biochemistry - Volume 46, Issue 9, September 2011, Pages 1879-1883
نویسندگان
Alvaro DÃaz-Barrera, Alberto Aguirre, Julio Berrios, Fernando Acevedo,