Effect of microwave irradiation on the structure of glucoamylase

► Structures changes of glucoamylase under microwave irradiation were analyzed. ► α-Helix, β-sheet, β-turn and random coil were determined by CD-spectroscopy. ► Synergistic effect between microwave and glucoamylase on starch hydrolysis. ► Mechanism of microwave in accelerating starch hydrolysis with glucoamylase.