Production of ortho-hydroxydaidzein derivatives by a recombinant strain of Pichia pastoris harboring a cytochrome P450 fusion gene

► CYP57B3 gene from Aspergillus oryzae was fused with the reductase domain of the CYP102A1 gene from Bacillus megaterium. ► Both the maximal production concentration and the conversion efficiency of 6-OHDe from daidzein in the present study are the highest values reported in the literatures to date. ► The present study is the first to demonstrate production of ortho-hydroxydaidzein derivatives using a fusion fungus cytochrome P450 enzyme.