Activity and stability of different immobilized preparations of recombinant E. coli cells containing ω-transaminase

► Principally different methods of whole-cell immobilization were compared. ► By flocculation with chitosan more than 60% activity was obtained at a very high cell loading of 3.2 g/g. ► More than 90% activity remained during eight repeated 1-h batches. ► Immobilized preparation useful in asymmetric synthesis of (S)-4-cyano-α-methylbenzylamine in repeated batches.