Operational stability of immobilized sucrose phosphorylase: Continuous production of α-glucose-1-phosphate at elevated temperatures

► Immobilization of sucrose phosphorylase enables its use at high temperatures. ► Heat-purification of the enzyme lowers the cost for industrial applications. ► The substrate sucrose stabilizes the biocatalyst in a packed-bed reactor. ► Glucose-1-phosphate is produced continuously at 60 °C with a high space-time yield. ► The product is efficiently purified and crystallized.