کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10236297 | 45143 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fed-batch production of recombinant fuculose-1-phosphate aldolase in E. coli
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Fed-batch production of recombinant fuculose-1-phosphate aldolase in E. coli Fed-batch production of recombinant fuculose-1-phosphate aldolase in E. coli](/preview/png/10236297.png)
چکیده انگلیسی
Escherichia coli strain XL1 blue (pTrcfuc) was used to overexpress fuculose-1-phosphate aldolase (Fuc-1-phosphate) in a fed-batch bioreactor operating at constant specific growth rate, 0.1 hâ1. Maximum dry cell weight concentration in the fermentor was 45 g dmâ3, over this biomass concentration oxygen became limiting. Once the recombinant strain behaviour was characterised under fixed growth conditions, optimal induction conditions to maximise the recombinant protein productivity in the system was studied. Time after induction to achieve maximum specific activity was around 11 h. Best results obtained were: specific activity of 1100 AU gâ1 DCW, enzyme concentration of 47,000 AU dmâ3 (around 3 g dmâ3) and global volumetric productivity of 1200 AU dmâ3 hâ1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 2, February 2005, Pages 707-716
Journal: Process Biochemistry - Volume 40, Issue 2, February 2005, Pages 707-716
نویسندگان
Olga Durany, Carles de Mas, Josep López-SantıÌn,