کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10268622 459675 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioelectrocatalytic and biosensing properties of horseradish peroxidase covalently immobilized on (3-aminopropyl)trimethoxysilane-modified titanate nanotubes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Bioelectrocatalytic and biosensing properties of horseradish peroxidase covalently immobilized on (3-aminopropyl)trimethoxysilane-modified titanate nanotubes
چکیده انگلیسی
Titanate nanotubes (TiNT) surface modified with (3-aminopropyl)trimethoxysilane were employed as a support for covalent immobilization of horseradish peroxidase (HRP) by using 1,4-benzoquinone as a coupling agent. Composite film-electrodes consisting of HRP-modified TiNT embedded into the porous carbon powder/Nafion matrix were fabricated and their applicability in direct bioelectrocatalytic reduction of H2O2 and H2O2 biosensing were investigated. An efficient direct electron transfer between the immobilized HRP molecules and the electrode was observed in the presence of H2O2 at potentials lower than 600 mV (vs. Hg/Hg2Cl2/3.5 M KCl). For the HRP-TiNT-modified electrodes polarized at 0 mV, a linear dependence of the bioelectrocatalytic current on the concentration of H2O2 was observed up to the concentration of H2O2 equal to 10 μM, with the sensitivity of (1.10 ± 0.01) AM−1 cm−2 and the detection limit of 35 nM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 56, Issue 27, 30 November 2011, Pages 9953-9960
نویسندگان
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