کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10268779 | 459675 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
3,4-Diaminobenzoic acid (DABA) as a redox label for electrochemical detection of single base mismatches
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 3,4-Diaminobenzoic acid (DABA) as a redox label for electrochemical detection of single base mismatches 3,4-Diaminobenzoic acid (DABA) as a redox label for electrochemical detection of single base mismatches](/preview/png/10268779.png)
چکیده انگلیسی
The present manuscript describes an electrochemical assay for detection of different types of single base mismatches (SBM) using 3,4-diaminobenzoic acid (DABA) as a new redox reporter. DABA is covalently attached to DNA, and its electrochemical response is followed. The present assay can overcome to two main problems of the hybridization biosensors, direct electron transfer of the redox reporter with the electrode surface and the positioning of the reporter before mismatch position. The introduced biosensor is able to discriminate complementary target and the targets including a single base mismatch even thermodynamically stable ones such as G-A and G-T.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 56, Issue 27, 30 November 2011, Pages 10264-10269
Journal: Electrochimica Acta - Volume 56, Issue 27, 30 November 2011, Pages 10264-10269
نویسندگان
Laleh Enayati Ahangar, Masoud A. Mehrgardi,