A novel l-lactate sensor based on enzyme electrode modified with ZnO nanoparticles and multiwall carbon nanotubes

► The novel multilayer structure (PDDA/LOD/ZnO/MWCNTs) provided a favorable microenvironment to keep the bioactivity of LOD. ► The electrode exhibited significant electrocatalysis to the oxidation and reduction of H2O2 and enhanced the responses. ► The electrode reduced the working potential, resulting in the enhancement of anti-interferences ability. ► LOD was strongly adsorbed on the surface of ZnO, which leaded to the good long-term stability. ► The prepared lactate biosensor exhibited the ideal Arrhenius temperature-dependant relationship from 10 to 50 °C.