کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10298826 | 539657 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellular resilience: 5-HT neurons in Tph2â/â mice retain normal firing behavior despite the lack of brain 5-HT
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
روانپزشکی بیولوژیکی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cellular resilience: 5-HT neurons in Tph2â/â mice retain normal firing behavior despite the lack of brain 5-HT Cellular resilience: 5-HT neurons in Tph2â/â mice retain normal firing behavior despite the lack of brain 5-HT](/preview/png/10298826.png)
چکیده انگلیسی
Considerable evidence links dysfunction of serotonin (5-hydroxytryptamine, 5-HT) transmission to neurodevelopmental and psychiatric disorders characterized by compromised "social" cognition and emotion regulation. It is well established that the brain 5-HT system is under autoregulatory control by its principal transmitter 5-HT via its effects on activity and expression of 5-HT system-related proteins. To examine whether 5-HT itself also has a crucial role in the acquisition and maintenance of characteristic rhythmic firing of 5-HT neurons, we compared their intrinsic electrophysiological properties in mice lacking brain 5-HT, i.e. tryptophan hydroxylase-2 null mice (Tph2â/â) and their littermates, Tph2+/â and Tph2+/+, by using whole-cell patch-clamp recordings in a brainstem slice preparation and single unit recording in anesthetized animals. We report that the active properties of dorsal raphe nucleus (DRN) 5-HT neurons in vivo (firing rate magnitude and variability; the presence of spike doublets) and in vitro (firing in response to depolarizing current pulses; action potential shape) as well as the resting membrane potential remained essentially unchanged across Tph2 genotypes. However, there were subtle differences in subthreshold properties, most notably, an approximately 25% higher input conductance in Tph2â/â mice compared with Tph2+/â and Tph2+/+ littermates (p<0.0001). This difference may at least in part be a consequence of slightly bigger size of the DRN 5-HT neurons in Tph2â/â mice (approximately 10%, p<0.0001). Taken together, these findings show that 5-HT neurons acquire and maintain their signature firing properties independently of the presence of their principal neurotransmitter 5-HT, displaying an unexpected functional resilience to complete brain 5-HT deficiency.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Neuropsychopharmacology - Volume 25, Issue 11, November 2015, Pages 2022-2035
Journal: European Neuropsychopharmacology - Volume 25, Issue 11, November 2015, Pages 2022-2035
نویسندگان
Alberto Montalbano, Jonas Waider, Mario Barbieri, Ozan Baytas, Klaus-Peter Lesch, Renato Corradetti, Boris Mlinar,