کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1036083 943875 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An improved PCR method for endogenous DNA retrieval in contaminated Neandertal samples based on the use of blocking primers
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد دانش مواد (عمومی)
پیش نمایش صفحه اول مقاله
An improved PCR method for endogenous DNA retrieval in contaminated Neandertal samples based on the use of blocking primers
چکیده انگلیسی

Neandertal skeletal remains are usually contaminated with modern human DNA derived from handling and washing of the specimens during excavation. Despite the fact that the distinct Neandertal haplotypes allow the design of specific primer pairs, for instance in most of the mitochondrial DNA (mtDNA) hypervariable region 1 (HVR1), the human contaminants can often outnumber the endogenous DNA, thus preventing a successful retrieval of Neandertal sequences. We have developed a novel PCR method, based on the use of blocking primers that preferentially bind to modern human contaminant DNA and block their amplification, and greatly improve the efficiency of Neandertal DNA retrieval. We tested the method in four El Sidrón Neandertal samples (two teeth and two bone fragments) with different contamination levels and taphonomic conditions, and we have been able to significantly increase the Neandertal yield from figures around 25.23% (5–69.6%) up to 90.18% (75.3–100%).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Archaeological Science - Volume 36, Issue 12, December 2009, Pages 2676–2679
نویسندگان
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