کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
103646 161396 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of multiplex assay with 16 SNPs on X chromosome for degraded samples
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of multiplex assay with 16 SNPs on X chromosome for degraded samples
چکیده انگلیسی

We selected 16 new X chromosomal SNPs (rs4827155, rs471205, rs7884160, rs16982419, rs985251, rs3813932, rs6630351, rs4132871, rs5966270, rs7471388, rs6641116, rs6521038, rs5990560, rs5959408, rs414960, and rs3006142) and developed the two X chromosomal SNPs Octaplex systems using multiplex single base extension reactions. To make the systems more useful for analyzing degraded DNA samples, we designed primers to render amplicons of 100 bp or shorter (shorter PCR products). Statistical analyses of the 16 SNPs indicated a high usefulness for the Japanese forensic practice. In addition, results of tests on degraded DNA confirm the usefulness of this technique in such samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Legal Medicine - Volume 14, Issue 1, January 2012, Pages 11–16
نویسندگان
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