Comparison of covalent immobilization of amylase on polystyrene pellets with pentaethylenehexamine and pentaethylene glycol spacers

► PS-PEHA-Ald and PS-PG-Ald pellets were studied as supports immobilizing amylase TAKA. ► The immobilization capacity of PS-PEHA-Ald was greater than that of PS-PG-Ald. ► PS-PG-Ald immobilized TAKA displayed a higher thermal stability. ► PS-PEHA-Ald immobilized TAKA was more stable in storage and reusability. ► The catalytic efficiency of TAKA was increased by the immobilization procedures.