Purification and biochemical characterization of a moderately halotolerant NADPH dependent xylose reductase from Debaryomyces nepalensis NCYC 3413

► Xylose reductase from Debaryomyces nepalensis was purified. ► Optimal pH and temperature for activity was 7.0 and 45 °C. ► Absolute requirement of NADPH for activity. ► Enzyme was found to be moderately halotolerant.