Saccharification of Kans grass using enzyme mixture from Trichoderma reesei for bioethanol production

► Kans grass as a novel lignocellulosic material was used as for bioethanol production. ► Optimization of on site production of crude cellulase enzyme from T. reesei. ► Acid pretreatment of Kans grass was followed by hydrolysis using crude enzyme. ► Resulted sugar after enzymatic hydrolysis was used for ethanol production by S. cerevisiae. ► On site produced crude enzyme may be more cost effective for bioethanol production.