Pretreatment of corn stover using low-moisture anhydrous ammonia (LMAA) process

► A new pretreatment method with anhydrous ammonia gas was investigated. ► Corn stover is ammoniated under nearly ambient conditions. ► Pretreated biomass was saccharified and fermented without an washing step. ► Conversion of C5 and C6 sugars into ethanol using recombinant strain.