کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10395200 | 888971 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of chemically defined media supporting high cell density growth of Ketogulonicigenium vulgare and Bacillus megaterium
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
The immediate precursor of L-ascorbic acid, or vitamin C, is 2-keto-l-gulonic acid (2-KLG). This is commonly produced commercially by Ketogulonicigenium vulgare and Bacillus megaterium, using corn steep liquor powder (CSLP) as an organic nitrogen source. In this study, the effects of the individual CSLP components (amino acids, vitamins, and metal elements) on 2-KLG production were evaluated, with the aim of developing a complete, chemically defined medium for 2-KLG production. Forty components of CSLP were analyzed, and key components were correlated to biomass, 2-KLG productivity, and consumption rate of L-sorbose. Glycine had the greatest effect, followed by serine, biotin, proline, nicotinic acid, and threonine. The combination of 0.28 g Lâ1 serine, 0.36 g Lâ1glycine, 0.18 g Lâ1 threonine, 0.28 g Lâ1proline, 0.19 g Lâ1nicotinic acid, and 0.62 mg Lâ1biotin in a chemically defined medium produced the highest maximum biomass concentration (4.2 Ã 109 cfu mLâ1), 2-KLG concentration (58 g Lâ1), and yield (0.76 g gâ1) after culturing for 28 h.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 102, Issue 7, April 2011, Pages 4807-4814
Journal: Bioresource Technology - Volume 102, Issue 7, April 2011, Pages 4807-4814
نویسندگان
Jing Zhang, Jingwen Zhou, Jie Liu, Kejie Chen, Liming Liu, Jian Chen,