کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
104010 | 161424 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of a rare mutation in a TH01 primer binding site
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
We experienced a difficult case of TH01 typing. Instability of TH01 allele 9.3 was observed using GenePrint STR System TH01. Allele dropout was observed when an AmpFâSTR Profiler Kit was subsequently used for confirmation of the TH01 type. Use of the PowerPlex 16 System made it possible to detect allele 9.3. As a result of sequencing, a single point mutation (G-to-A transition) located 37 bases upstream of the first TCAT motif of the repeat region was identified as the cause of the allele dropout during use of the AmpFâSTR Profiler Kit. This mutation was located at the 3â² end of the forward primers of the AmpFâSTR Profiler Kit and GenePrint STR System TH01.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Legal Medicine - Volume 9, Issue 6, November 2007, Pages 289-292
Journal: Legal Medicine - Volume 9, Issue 6, November 2007, Pages 289-292
نویسندگان
Tomohiro Takayama, Naoki Takada, Rie Suzuki, Shunsuke Nagaoka, Yoshihisa Watanabe,