High-performance bioanode based on the composite of CNTs-immobilized mediator and silk film-immobilized glucose oxidase for glucose/O2 biofuel cells

► A high-performance bioanode was developed for glucose/O2 biofuel cell (BFC). ► A composite of CNTs-immobilized mediator and silk film-immobilized glucose oxidase. ► The bioanode exhibited good catalytic activity and excellent stability. ► BFCs with open circuit potential of 0.48 V and maximum power density of 50.70 μW cm−2.