کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429347 | 909788 | 2012 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Indirect electrocatalytic determination of choline by monitoring hydrogen peroxide at the choline oxidase-prussian blue modified iron phosphate nanostructures
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Choline, as a marker of cholinergic activity in brain tissue, is very important in biological and clinical analysis, especially in the clinical detection of the neurodegenerative disorders disease. This work presents an electrochemical approach for the detection of choline based on prussian blue modified iron phosphate nanostructures (PB-FePO4). The obtained nanostructures showed a good catalysis toward the electroreduction of H2O2, and an amperometric choline biosensor was developed by immobilizing choline oxidase on the PB-FePO4 nanostructures. The biosensor exhibited a rapid response (ca. 2 s), low detection limit (0.4 ± 0.05 μM), wide linear range (2 μM to 3.2 mM), high sensitivity (â¼75.2 μA mMâ1 cmâ2), as well as good stability and repeatability. In addition, the common interfering species, such as ascorbic acid, uric acid and 4-acetamidophenol did not cause obvious interference due to the low detection potential (â0.05 V versus saturated calomel electrode). This nanostructure could be used as a promise platform for the construction of other oxidase-based biosensors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 31, Issue 1, 15 January 2012, Pages 244-250
Journal: Biosensors and Bioelectronics - Volume 31, Issue 1, 15 January 2012, Pages 244-250
نویسندگان
Hui Zhang, Yajing Yin, Ping Wu, Chenxin Cai,