A novel combined thermometric and amperometric biosensor for lactose determination based on immobilised cellobiose dehydrogenase

► Combined thermometric/amperometric biosensor based on immobilised CDH is proposed. ► The sensor was applied for the determination of lactose in dairy milk samples. ► No sample preparation except dilution with buffer was needed. ► The linear response for lactose was obtained between 0.05 mM and 30 mM. ► For a set of more than 500 samples an R.S.D. of less than 10% was achieved.