DNA-functionalized biosensor for riboflavin based electrochemical interaction on pretreated pencil graphite electrode

► We introduced a biosensor based on interaction of riboflavin at a salmon sperm ds-DNA-modified pencil graphite electrode (DNA-PGE) and pretreated pencil graphite electrode for determination of riboflavin. We investigated the mechanism of the interaction and confirmed by differential pulse voltammetry using two different interaction processes, on the PGE surface and in the solution phase. The biosensor was applied and validated for the analysis of riboflavin in pharmaceutical and urine samples.