Novel electrochemical catalysis as signal amplified strategy for label-free detection of neuron-specific enolase

► We developed a novel signal amplified strategy by electrochemical catalysis for label-free detection of NSE. ► Gold nanoparticles functionalized graphene nanosheets (Au-Gra) as antibody immobilization. ► The signal was amplified according to electrocatalytic oxidation of l-dopamine (DA) by nickel hexacyanoferrates nanoparticles (NiHCFNPs) which avoiding enzyme inactivation. ► Analysis of real samples and method comparison.