Simultaneous detection of multiple mutations in epidermal growth factor receptor based on fluorescence quenching of quantum dots

► We develop a simultaneous detection method for multiple mutations. ► We detect two common mutations in the epidermal growth factor receptor gene. ► Fluorescence quenching due to aggregation of quantum dots is used for detection. ► This enables a simultaneous detection of mutations in exon 19 and 21 of EGFR gene.