LSPR biomolecular assay with high sensitivity induced by aptamer-antigen-antibody sandwich complex

► The sandwich complex enhanced the LSPR peak shift more than 150%. ► The use of DNA aptamers as thrombin capture units makes the sensor reusable. ► Calibration curve and sample detection were achieved by a single nanoparticle. ► An averaged LOD of 1.6 pM was achieved for thrombin detection.