کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429432 | 909789 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A biomimetic sensor for the determination of extracellular O2â using synthesized Mn-TPAA on TiO2 nanoneedle film
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
By immobilizing synthesized Mn-TPAA (TPAA = tris[2-[N-(2-pyridylmethyl) amino] ethyl] amine) on TiO2 nanoneedle surface, a biosensor for superoxide ion (O2â) has been developed and applied for determination of O2â released from living cells. Direct electron transfer of Mn-TPAA is realized with a formal redox potential (E°â²) falling in the range of the E°Ⲡvalues of the redox couples O2/O2â and O2â/H2O2. This suggests that Mn-TPAA on TiO2 films is electrochemically active and capable of thermodynamically mediating both the oxidation of O2â to O2 and the reduction of O2â to H2O2. Therefore, Mn-TPAA immobilized on the TiO2 films can be used electrochemically for determination of O2â due to its electrochemical activities and biomimetic catalytic activities like superoxide dismutase (SOD) toward O2â. The present biomimetic O2â sensor shows high selectivity at the low working potential of 0 V vs. Ag|AgCl, a wide linear range from 10â7 M to 10â4 M and a quick response time within 6 s. By taking advantage of the developed method and the properties of biomimetic SOD themselves, we have realized the real-time monitoring of O2â concentration released from living cells and investigated the relationship between the concentration changes of O2â and intracelluar Ca2+, which may gain additional insights on the reactive oxygen species (ROS) signal transduction and other physiological and pathological events.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 29, Issue 1, 15 November 2011, Pages 189-194
Journal: Biosensors and Bioelectronics - Volume 29, Issue 1, 15 November 2011, Pages 189-194
نویسندگان
Yongping Luo, Guoqi Hu, Anwei Zhu, Biao Kong, Zhen Wang, Chunyuan Liu, Yang Tian,