کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429530 | 909796 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ATP microelectrode biosensor for stable long-term in vitro monitoring from gastrointestinal tissue
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have developed a stable and selective ATP biosensor for long-term in vitro tissue monitoring. The electrode was fabricated by entrapping glucose oxidase (GOx) and hexokinase (HEX) in a poly-phenol film on a Pt microelectrode. The biosensor was stable to a fixed concentration of glucose for over 20 min and had a limit of detection of 9.9 ± 3.2 nM, with a sensitivity of 45.8 ± 1.22 pA μMâ1. Most significantly of all, the response on the ATP biosensor did not alter in the presence of 1 mM ascorbic acid, 5 μM dopamine, 5 μM serotonin, 5 μM ADP and 5 μM AMP. The ATP biosensor was also shown to have excellent stability over 7 days, and showed only a 23.92 ± 3.55% loss in sensitivity. The ATP biosensor was utilised for the in vitro detection of ATP from gastrointestinal tissue. The ATP biosensor response was stable for 5 h during in vitro recordings from ileum tissue. ATP release was shown to be greater from the mucosal surface in the ileum compared to the colon.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 26, Issue 6, 15 February 2011, Pages 2890-2896
Journal: Biosensors and Bioelectronics - Volume 26, Issue 6, 15 February 2011, Pages 2890-2896
نویسندگان
Bhavik Anil Patel, Michelle Rogers, Talia Wieder, Danny O'Hare, Martyn G. Boutelle,