کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429557 | 909796 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An aptamer-based chromatographic strip assay for sensitive toxin semi-quantitative detection
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
An aptamer-based chromatographic strip assay method for rapid toxin detection was developed. The aptamer-based strip assay was based on the competition for the aptamer between ochratoxin A and DNA probes. The sensing results indicated that the sensitivity of the aptamer-based strip was better than that of conventional antibody-based strips. The visual limit of detection of the strip for qualitative detection was 1Â ng/mL while the LOD for semi-quantitative detection could down to 0.18Â ng/mL by using scanning reader. The recoveries of test samples were from 96% to 110%. All detections could be achieved in less than 10Â min, indicating that the aptamer-based strip could be a potential useful tool for rapid on-site detections.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 26, Issue 6, 15 February 2011, Pages 3059-3062
Journal: Biosensors and Bioelectronics - Volume 26, Issue 6, 15 February 2011, Pages 3059-3062
نویسندگان
Libing Wang, Wenwei Ma, Wei Chen, Liqiang Liu, Wei Ma, Yingyue Zhu, Liguang Xu, Hua Kuang, Chuanlai Xu,