کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429594 | 909845 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2â²-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2â²-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2â²-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material](/preview/png/10429594.png)
چکیده انگلیسی
An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2â²-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol-gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol-gel film to provide the microenvironment for retaining the activity of enzyme and immobilize tris(2,2â²-bipyridyl)ruthenium (II). The design was simpler than the previous two-layer format. The experimental conditions, such as scan rate, pH and concentration of the cofactor were investigated. The intensity of electrogenerated chemiluminescence increased linearly with ethanol concentration from 2.5Â ÃÂ 10â5 to 5.0Â ÃÂ 10â2Â M and detection limit was 1.0Â ÃÂ 10â5Â M. The prepared biosensor exhibited high sensitivity, wide linear range and good stability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 21, Issue 3, 15 September 2005, Pages 455-461
Journal: Biosensors and Bioelectronics - Volume 21, Issue 3, 15 September 2005, Pages 455-461
نویسندگان
Zhiai Xu, Zhihui Guo, Shaojun Dong,