کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429798 | 909866 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biosensing and monitoring of cell populations using the hydrogel bacterial microchip
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کلمات کلیدی
PBSHTSIPTGeGFParsenite - آرسنیتAntibiotics - آنتی بیوتیک هاisopropyl β-D-thiogalactopyranoside - ایزوپروپیل β-D-thiogalactopyranosideBiosensor - بیوسنسورImmobilized cells - سلول های ایمن شدهhigh throughput screening - غربالگری بالاPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Advanced development of the hydrogel bacterial microchip (HBMChip) technique is proposed. The microchip represents an array of hemispherical gel elements 0.3-60Â nl in volume attached to hydrophobic glass surface and containing live immobilized microbial cells. Separate gel elements contain each up to 105Â cells and retain them inside even while the cells are dividing. Porous structure of the gel provides easy access of nutrients and tested substances to the immobilized cells. Optical signals from the cells are easily measurable and allow monitoring of intracellular metabolism using vital fluorescent stains or engineered constructs encoding bioluminescent or fluorescent reporters. Two possible application modes of the HBMChip have been investigated, i.e. the observation of bacteria and biosensing. The dynamics of nucleic acids synthesis in growing E. coli cells has been analyzed using vital fluorescent stain SYTO 9. A special function has been suggested for evaluation of the cell growth parameters. Biosensing properties of the HBMChip have been illustrated by quantitative analysis of antibiotics and the detection of sodium meta-arsenite.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 20, Issue 9, 15 March 2005, Pages 1860-1865
Journal: Biosensors and Bioelectronics - Volume 20, Issue 9, 15 March 2005, Pages 1860-1865
نویسندگان
D.O. Fesenko, T.V. Nasedkina, D.V. Prokopenko, A.D. Mirzabekov,