کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10435112 910840 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Primary cilia act as mechanosensors during bone healing around an implant
ترجمه فارسی عنوان
در هنگام استخوان سازی استخوان در اطراف یک ایمپلنت، ایمونولوژی اولیه به عنوان مکانیسنسور عمل می کند
کلمات کلیدی
مکانیک انتقال، استئوبلاست، ایمپلنت: پوکی استخوان،
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی پزشکی
چکیده انگلیسی
The primary cilium is an organelle that senses cues in a cell's local environment. Some of these cues constitute molecular signals; here, we investigate the extent to which primary cilia can also sense mechanical stimuli. We used a conditional approach to delete Kif3a in pre-osteoblasts and then employed a motion device that generated a spatial distribution of strain around an intra-osseous implant positioned in the mouse tibia. We correlated interfacial strain fields with cell behaviors ranging from proliferation through all stages of osteogenic differentiation. We found that peri-implant cells in the Col1Cre;Kif3afl/fl mice were unable to proliferate in response to a mechanical stimulus, failed to deposit and then orient collagen fibers to the strain fields caused by implant displacement, and failed to differentiate into bone-forming osteoblasts. Collectively, these data demonstrate that the lack of a functioning primary cilium blunts the normal response of a cell to a defined mechanical stimulus. The ability to manipulate the genetic background of peri-implant cells within the context of a whole, living tissue provides a rare opportunity to explore mechanotransduction from a multi-scale perspective.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Medical Engineering & Physics - Volume 35, Issue 3, March 2013, Pages 392-402
نویسندگان
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