A new miniSTR heptaplex system for genetic fingerprinting of ancient DNA from archaeological human bone

► We developed a short amplicon tandem repeat (miniSTR) heptaplex PCR system. ► The system was designed with respect to the special demands of degraded DNA. ► It produces reproducibly full STR profiles down to a concentration of 0.06 ng DNA. ► The system is a reliable and inexpensive tool for genetic fingerprinting on aDNA. ► The system is suitable for kinship reconstruction in archeological burial contexts.