Detection of Helicobacter pylori DNA in preliminary stage gastric cancer cells

SummaryAimsHelicobacter pylori (HP) DNA was assayed using a newly fabricated voltammetric handheld circuit equipped with an interfaced graphite pencil electrode (PE) sensor.MethodsUnder optimum analytical parameters, the square- wave (SW) stripping detection range attained was 0.1–0.7 mg/L.ResultsA relative standard deviation of 1.68% (RSD, n = 5) was observed at a 3.2mg/L HP DNA within a 90s accumulation time. Under these conditions, the analytical detection limit approached 0.07 mg/L.ConclusionsThe developed methods can be applied to HP assays of preliminary stage gastric cancer patient tissues.