کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10529393 | 960802 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spectrophotometric determination of sulfhydryl concentration on the surface of thiol-modified chromatographic silica particles using 2,2â²-dipyridyl disulfide reagent
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This article describes a straightforward method for the quantification of thiol groups on the surface of chromatographic particles such as 3-mercaptopropyl silica gel. The method is based on a thiol-disulfide exchange reaction with 2,2â²-dipyridyl disulfide (DPDS) reagent and spectrophotometric or HPLC-UV determination of the liberated 2-pyridyl thione. Linearity was obtained over the sulfhydryl concentration range of 0.02-0.5 μmol mlâ1 for the spectrophotometric determination and 0.2-5 μmol mlâ1 for the HPLC-UV measurement, with r2 values of 0.9975 and 0.9981, respectively. The results were validated using elemental analysis as independent control method. The agreement between the results of both methods indicates that the sulfur of the thiol-modified silica particles is available as reactive sulfhydryls. The repeatability study indicated a higher precision for the DPDS method compared to the elemental analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 533, Issue 2, 28 March 2005, Pages 179-183
Journal: Analytica Chimica Acta - Volume 533, Issue 2, 28 March 2005, Pages 179-183
نویسندگان
R. Nogueira, M. Lämmerhofer, N.M. Maier, W. Lindner,