کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1162743 1490898 2016 6 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
A new sample preparation and separation combination for precise, accurate, rapid, and simultaneous determination of vitamins B1, B2, B3, B5, B6, B7, and B9 in infant formula and related nutritionals by LC-MS/MS
ترجمه فارسی عنوان
آماده سازی و جداسازی نمونه برای تعیین دقیق، دقیق، سریع و همزمان ویتامین B1، B2، B3، B5، B6، B7 و B9 در شیر مادر و مواد غذایی مرتبط با LC-MS / MS
کلمات کلیدی
ویتامین های محلول در آب؛ LC-MS / MS؛ طیف سنجی جرمی؛ کروماتوگرافی مایع؛ شیر خشک نوزاد
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Fast, accurate, and precise water soluble vitamin determination in a wide range of infant formula and nutritional products.
• Improvements in sample preparation and separation conditions are key contributors to method performance.
• The method was validated in eleven different infant formula and related matrixes with excellent demonstrated accuracy and precision.

An improved method was developed for simultaneous determination of the fortified forms of thiamine (B1), riboflavin (B2), nicotinamide and nicotinic acid (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7), and folic acid (B9) in infant formulas and related nutritionals. The method employed a simple, effective, and rapid sample preparation followed by liquid chromatography tandem mass spectrometry (LC-MS/MS). It improved upon previous methodologies by offering facile and rugged sample preparation with improved chromatographic conditions, which culminated in a highly accurate and precise method for water-soluble vitamin determination in a wide range of formulas. The method was validated over six days in ten unique matrices with two analysts and on instruments in two different labs. Intermediate precision averaged 3.4 ± 2.6% relative standard deviation and over-spike recovery averaged 100.2 ± 2.4% (n = 160). Due to refinements in sample preparation, the method had high sample throughput capacity.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 934, 31 August 2016, Pages 180–185
نویسندگان
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