کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10536750 | 962600 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure and dynamics of the retro-form of the bacteriophage T5 endolysin
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کلمات کلیدی
SSDDSSIDPDPCHigh-resolution NMR - NMR با رزولوشن بالاIntrinsic disorder - اختلال ذهنیSpin diffusion - انتشار اسپینEndolysin - اندولیسینFlexibility - انعطاف پذیریProtein folding - تاشدگی پروتئینDodecylphosphocholine - دودهیل فسفو هولینProtein dynamics - دینامیک پروتئینIntrinsically disordered protein - پروتئین ناسازگار ذاتی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Using high-resolution NMR spectroscopy we conducted a comparative analysis of the structural and dynamic properties of the bacteriophage T5 endolysin (EndoT5) and its retro-form; i.e., a protein with the reversed direction of the polypeptide chain (R-EndoT5). We show that structurally, retro-form can be described as the molten globule-like polypeptide that is easily able to form large oligomers and aggregates. To avoid complications associated with this high aggregation propensity of the retro protein, we compared EndoT5 and R-EndoT5 in the presence of strong denaturants. This analysis revealed that these two proteins possess different internal dynamics in solutions containing 8Â M urea, with the retro-form being characterized by larger dimensions and slower internal dynamics. We also show that in the absence of denaturant, both forms of the bacteriophage T5 endolysin are able to interact with micelles formed by the zwitterionic detergent dodecylphosphocholine (DPC), and that the formation of the protein-micelle complexes leads to the significant structural rearrangement of polypeptide chain and to the formation of stable hydrophobic core in the R-Endo T5.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1864, Issue 10, October 2016, Pages 1281-1291
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1864, Issue 10, October 2016, Pages 1281-1291
نویسندگان
Victor P. Kutyshenko, Galina V. Mikoulinskaia, Nikolai V. Molochkov, Dmitry A. Prokhorov, Sergei A. Taran, Vladimir N. Uversky,