کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10536777 | 962605 | 2015 | 47 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Time-series analysis of the transcriptome and proteome of Escherichia coli upon glucose repression
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کلمات کلیدی
CCRFDRSmall regulatory RNAPTSSubstrate-level phosphorylationSLPMudPITAUC - AUCTime-series analysis - تجزیه و تحلیل سری زمانProteomics analysis - تجزیه پروتئومیکسStem - ساقهCarbon catabolite repression - سرکوب کاتابولیت کربنphosphotransferase system - سیستم فسفات ترانسفرازMultidimensional protein identification technology - فن آوری شناسایی پروتئین چندتاییarea under the curve - منطقه تحت منحنیfalse discovery rate - میزان کشف کاذبStrong cation exchange chromatography - کروماتوگرافی تبادل کاتیونی قویPost-transcriptional control - کنترل Post-transcriptional
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Time-series transcript- and protein-profiles were measured upon initiation of carbon catabolite repression in Escherichia coli, in order to investigate the extent of post-transcriptional control in this prototypical response. A glucose-limited chemostat culture was used as the CCR-free reference condition. Stopping the pump and simultaneously adding a pulse of glucose, that saturated the cells for at least 1Â h, was used to initiate the glucose response. Samples were collected and subjected to quantitative time-series analysis of both the transcriptome (using microarray analysis) and the proteome (through a combination of 15N-metabolic labeling and mass spectrometry). Changes in the transcriptome and corresponding proteome were analyzed using statistical procedures designed specifically for time-series data. By comparison of the two sets of data, a total of 96 genes were identified that are post-transcriptionally regulated. This gene list provides candidates for future in-depth investigation of the molecular mechanisms involved in post-transcriptional regulation during carbon catabolite repression in E. coli, like the involvement of small RNAs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1854, Issue 10, Part A, October 2015, Pages 1269-1279
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1854, Issue 10, Part A, October 2015, Pages 1269-1279
نویسندگان
Orawan Borirak, Matthew D. Rolfe, Leo J. de Koning, Huub C.J. Hoefsloot, Martijn Bekker, Henk L. Dekker, Winfried Roseboom, Jeffrey Green, Chris G. de Koster, Klaas J. Hellingwerf,